Bax translocation precedes mitochondrial fragmentation during staurosporine treatment
Clone 10 cells express a low level of GFP-Bax, which allows monitoring early MAC formation, corresponding to Bax translocation. These cells were transiently transfected to express mtRFP (red) targeted to mitochondria to visualize their morphology. Images were taken every 20 minutes of cells treated with 1 µM staurosporine for a time-lapse fluorescence video. Merged images show translocation of Bax via green fluorescence as it changes from diffuse in the cytosol to punctate in mitochondria in panel B (^) and red mitochondria fragment in panel C. Bax translocation (green, ^) followed mitochondrial fragmentation (red) by 54±19 min. Scale bar is 20 µm in C for top of each panel and 5 µm in panel A for expanded region.