Research>Shared Scientific Core Resources

Biological Analytics

Equipment and Fees

Azure Biosystems C400 Imaging System

Azure Biosystems C400 Imaging System

*requires training prior to scheduling use

NYU $5/hr; External Academic: $10/hr; External Contract: $15/hr

   

gel documentation

BioRad ChemiDoc gel documentation

* Requires training prior to scheduling use.

NYU: $5/hr; External Academic: $10/hr; External Contract: $15/hr

Please visit the BioRad website for more information.

 

plate reader

Molecular Devices Plate Reader

* Requires training prior to scheduling use.

NYU: $25/hr; External Academic: $40/hr; External Contract: $200/hr

  • Dual monochromator spectrophotometer with five detection modes in one platform: UV-visible Absorbance (Abs); Fluorescence Intensity (FI); Time-Resolved Fluorescence (TRF); Fluorescence Polarization (FP); Glow Luminescence (Lumi).
  • Reads 96 and 384 well microplates and transforms each well in a microplate to a fixed optical pathlength cuvette. Temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.
  • For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET and TR-FRET assays.
  • For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.
  • The SpectraMax M5 Microplate Reader uses SoftMax® Pro. Data Acquisition & Analysis Software. Among the various ready-to-read protocols, users will find pre-written SpectraDrop™ Plate protocols enabling Micro Volume applications measurement.
  • Precision Optics and Detectors: The SpectraMax M5 Microplate Reader utilizes several key technologies to ensure optimal sensitivity for all modes.
  • Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
  • Dual photomultiplier tubes (PMTs) provide detection flexibility to the widest variety of assays.
  • Reference diodes automatically adjust to slight fluctuations in excitation intensity to reduce measurement noise.
  • Absorbance applications are enhanced using top-quality UV grade fibers to provide strong light transmission in the lowest wavelengths.
msd plate reader scanner

Meso Scale Discovery (MSD) QuickPlex SQ120 Plate Reader/Scanner

* Training is required prior to scheduling

Initial Training: $100/hr
Trained users operating independently: $50/hr
Assisted operation: $100/hr
$25/hr: Malamud Laboratory & the Department of Orthopaedic Surgery

  • The Quickplex SQ120 is the newest multiplexing instrument from MSD, offering high performance, reliable, electrochemiluminescence immunoassays and reads all 96well SECTOR plates
  • System provides the combination of rapid read times with the ability to perform multiple, simultaneous tests on a single sample.
  • System comprises proprietary arrays coupled with electrochemiluminescence detection which offers a unique combination of sensitivity and a 5 log dynamic range.
  • The 10 spot arrays allow for parallel processing of biomarkers using minimal amounts of sample.
  • Current assays include human, mouse, rat, non-human primate, canine biomarkers as well as a "naked" plate format that allows the user to develop their own assay.
  • System reads the QUICKPLEX plates which are intended for user-developed assays and offered in both coated and uncoated formats.
  • The read time for a QUICKPLEX plate is approximately three minutes.
  • Free analysis software can be downloaded for personal use on PC type computers only.
  • Information on available assays, literature etc. can be found on the company’s web site.

Detection Technology: Electrochemiluminescence (ECL)

Detection System: Cooled scientific-grade CCD camera and precision custom lens

Multiplexing Capability: Up to 10 assay spots per well

Assay Plate Compatibility: MSD SECTOR and QUICKPLEX plates

Sensitivity: 0.05 to 1 pg/mL for specific assays

Dynamic Range: > 105

Read Volume: 150 µL

Operating Temperature Range: 59° to 90°F (15° to 32°C)

Measured Sound Level: 59 dbA

nanodrop

Nanodrop 2001

* Requires training prior to scheduling use.

NYU: $6/hr; External Academic: $20/hr; External Contract: $100/hr

  • The NanoDrop 2000 is a micro-volume spectrophotometer for measuring DNA, RNA, and protein. The NanoDrop 2000 accurately measures samples as small as 0.5 µL, and reports sample concentration, purity ratios, and full spectral data.