Mitochondrial Fragmentation Dynamics

Mitochondrial fragmentation is rapidly induced by rotenone. Fluorescence images of three cells are shown after staining with mitotracker red to visualize the mitochondrial network (red) before (0 min) and after addition of 200 nM rotenone. Normal mitochondria are elongated with multiple shapes and form a network (0 min). Rotenone induces a rapid fragmentation of the network after 20 minutes which continues to deteriorate with time as mitochondria become small spheres. Frames acquired every 20 min. and cells are dead by 18 hours when video ends.