Mitochondrial Permeabilization

MEFs were co-transfected with plasmids encoding GFP targeted to the matrix (mtGFP) to visualize mitochondrial morphology and SMAC-Cherry targeted to the intermembrane space to see outer membrane permeabilization. Time-lapse video of merged fluorescence images show two cells stained with Hoechst and treated with 500 nM staurosporine for 2-4 hours. Mitochondria turn from yellow to green as SMAC-Cherry is released in a wave across both cells. Mitochondria then fragment, which is followed by rounding up of the cells and nuclear condensation. Scale Bar is 10 ?M for all frames.